Research in Medicine

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Paydarfar S, Hashemi A, Abbsi A, bandehpor M, Bahtiari A, Tajik F et al . Investigation of preformed design Acinetobacter Baumannii outer membrane protein A peptide vaccine candidate in reaction with serum antibodies from ICU staffs members. Research in Medicine 2020; 44 (4) :607-612
URL: http://pejouhesh.sbmu.ac.ir/article-1-2116-en.html

Background: The staff members in ICU are the major carriers with different devices such as catheters.The aim of the present study was to investigate the antibody reaction against immunogenic peptides of ompA designed as a candidate vaccine Against acinetobacter baumannii.
Materials and method: A total of 62 serum samples were obtained from exposed ICU staffs in Tehran Firoozabadi and Dezful Ganjavian hospitals and non-exposed individuals and patients suffering from SLE. In order to increase specificity and to decrease non-specific anti body, all the serums were preadsorped with E-coli Lysate. In order to determine positive reaction of samples with Acinetobacter baumannii lysate due to detection of existence of antibodies against Acinetobacter baumannii, ELISA technique was used. 
Results: The results showed that out of 33 samples of exposed serum, 75% was positive. Also, from among 22 samples of non-exposed individuals, 27% was positive. Strongly positive serum with lysates were reacted with only peptide 5 of ompA Acinetobacter baumannii. 
Conclusion: Obtained results showed that not only exposed samples had a lot more Ab titre in comparison
to non-exposed sample, but these groups had also significant higher titer of Abs against peptide 5. In other words, it can be concluded that the colonization of Acinetobacter baumannii in population is growing year by year. More research should be carried out to demonstrate the exact role of antibody against Acinetobacter baumannii and immunologic peptides.