Volume 48, Issue 2 (8-2024)
Research in Medicine 2024, 48(2): 12-26 |
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Ethics code: IR.IAU.MSHD.REC.1402.082
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Fazeli Z, Neamati A, Karimi E. Investigating the Effect of Zein- Betacyclodextrin Nanoparticles Loaded with Naringin on MCF7 Breast Cancer Cell Line. Research in Medicine 2024; 48 (2) :12-26
URL: http://pejouhesh.sbmu.ac.ir/article-1-3325-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3325-en.html
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , neamati.ali@gmail.com
Abstract: (199 Views)
Background and Aim: Despite recent advances in diagnosis and treatment, breast cancer is still the first cause of death for women worldwide. Today, zein is used as a widely used component in drug delivery systems due to its excellent hydrophobicity, biodegradability, biological adhesion and economic characteristics. This study was conducted with the aim of investigating the effect of zein- beta- cyclodextrin nanoparticles loaded with naringin on the expression of Cas9 and Bcl2 genes in MCF7 cell line.
Methods: In this experimental study, the size, surface morphology, and chemical structure of the synthesized β-CD-zein nanoparticles were determined by dynamic light scattering (DLS), scanning electron microscopy, and Fourier transform infrared spectroscopy (FT-IR), respectively. MCF7 cells were treated with different concentrations of zein-beta- cyclodextrin nanoparticles loaded with naringin (15.6, 31.2, 62.5, 125, 250 and 500 μg/ ml) for 24 hours and then the toxicity effect was evaluated using the MTT test. DAPI staining was performed to investigate the induction of apoptosis, and a flow cytometry assay was performed to evaluate the effects of β-CD- zein on cell viability, migration, and expression of genes related to migration and apoptosis. Finally, the expression of the Cas9 and Bcl2 genes compared to the GAPDH gene was checked by the real-time method. The results of these surveys were analyzed with SPSS software and Tukey's post hoc test.
Results: The results showed that the nanoparticle was synthesized with a size of 144 nm, zeta potential of -16.2 mV, uniform shape and expected structure. The dispersion index of PDI was calculated as 0.17. Also, with increasing the concentration of nanoparticles loaded with naringin, the survival rate of cells decreased significantly compared to the control. Moreover, Real-time PCR showed that the expression of Cas9 and Bcl2 gene in 24 hours under the influence of different concentrations of zein- beta- cyclodextrin nanoparticles loaded with naringin (IC50 with a concentration of 62.5 μg/ ml) significantly increased and decreased, respectively (P < 0.01.) compared to the control group.
Conclusion: Based on the findings of this research, the combination of zein- beta cyclodextrin loaded with naringin is effective against breast cancer cells and can be studied as a suitable candidate for breast cancer treatment.
Methods: In this experimental study, the size, surface morphology, and chemical structure of the synthesized β-CD-zein nanoparticles were determined by dynamic light scattering (DLS), scanning electron microscopy, and Fourier transform infrared spectroscopy (FT-IR), respectively. MCF7 cells were treated with different concentrations of zein-beta- cyclodextrin nanoparticles loaded with naringin (15.6, 31.2, 62.5, 125, 250 and 500 μg/ ml) for 24 hours and then the toxicity effect was evaluated using the MTT test. DAPI staining was performed to investigate the induction of apoptosis, and a flow cytometry assay was performed to evaluate the effects of β-CD- zein on cell viability, migration, and expression of genes related to migration and apoptosis. Finally, the expression of the Cas9 and Bcl2 genes compared to the GAPDH gene was checked by the real-time method. The results of these surveys were analyzed with SPSS software and Tukey's post hoc test.
Results: The results showed that the nanoparticle was synthesized with a size of 144 nm, zeta potential of -16.2 mV, uniform shape and expected structure. The dispersion index of PDI was calculated as 0.17. Also, with increasing the concentration of nanoparticles loaded with naringin, the survival rate of cells decreased significantly compared to the control. Moreover, Real-time PCR showed that the expression of Cas9 and Bcl2 gene in 24 hours under the influence of different concentrations of zein- beta- cyclodextrin nanoparticles loaded with naringin (IC50 with a concentration of 62.5 μg/ ml) significantly increased and decreased, respectively (P < 0.01.) compared to the control group.
Conclusion: Based on the findings of this research, the combination of zein- beta cyclodextrin loaded with naringin is effective against breast cancer cells and can be studied as a suitable candidate for breast cancer treatment.
Type of Study: Original |
Subject:
Genetic
Received: 2023/09/2 | Accepted: 2024/05/26 | Published: 2024/09/16
Received: 2023/09/2 | Accepted: 2024/05/26 | Published: 2024/09/16
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