Volume 30, Issue 3 (9-2006)                   Research in Medicine 2006, 30(3): 245-252 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nejad Moghaddam M, Modarresi M, BabaShamsi M, Chamankhah M. Streptokinase: extraction, cloning and overexpression of recombinant. Research in Medicine 2006; 30 (3) :245-252
URL: http://pejouhesh.sbmu.ac.ir/article-1-321-en.html
Streptokinase: extraction, cloning and overexpression of recombinant
MR1 Nejad Moghaddam , MH2 Modarresi , M3 BabaShamsi , M3 Chamankhah
, babashams@avesina.ac.ir
Abstract:   (22386 Views)
Abstract: Background: Streptokinase has been widely prescribed as a fibrinolytic agent for myocardial infarction. Simple structural characteristics of this protein have provided techniques for production of different recombinant types of this protein. The present study was designed to prepare equisimilisH46A subtype of streptokinase in our country. Materials and methods: Having extracted the DNA, the streptokinase gene was replicated and cloned in pGEX-4T-2. The recombinant product was transformed in BL21(DE3)plysS'. Then the recombinant streptokinase expression and performance was assessed by lasic densitometry and special test for S2251 substrate. Results: Use of a restriction enzyme for both sides of a gene may facilitate its cloning in different expressive carriers. Expression rate of recombinant protein (45%) confirmed successful cloning. Conclusions: Use of pGEX-4T-2 carrier was not only associated with active recombinant streptokinase production, added GST to its amine ending that could facilitate purification process.
Keywords: pGEX-4T-2, Streptokinase, Cloning, Recombinant protein, Purification
Full-Text [PDF 403 kb]   (9006 Downloads)    
Type of Study: Original | Subject: Interdisciplinary (Educational Management, Educational research, Statistics, Medical education
Received: 2007/08/4 | Published: 2006/09/15
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This Journal is licensed under a Creative Commons Attribution 4.0 International License  | Research in Medicine

Designed & Developed by : Yektaweb