Volume 50, Issue 1 (5-2026)
Research in Medicine 2026, 50(1): 0-0 |
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Ethics code: IR.IAU.SHK.REC.1404.176
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Haghshenas S, Peymani M. Evaluation of TENT4A and TENT4B Genes in Colorectal Cancer: Signaling Pathway Analysis and Expression Evaluation in Cell Lines. Research in Medicine 2026; 50 (1)
URL: http://pejouhesh.sbmu.ac.ir/article-1-3521-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3521-en.html
Department of Biology, Shk.C, Islamic Azad University, Shahrekord, Iran. , m.peymani@iau.ac.ir
Abstract: (39 Views)
Background and Aim: Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide, and identifying novel molecular biomarkers is essential for early diagnosis and prognostic prediction. TENT4A and TENT4B belong to the family of non- canonical poly (A) polymerases involved in RNA stability and translational regulation. This study aimed to investigate the expression patterns of these genes and their associated signaling pathways in colorectal cancer.
Methods: This descriptive- analytical study was conducted using RNA sequencing (RNA-Seq) data obtained from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) between tumor and normal tissues were identified using the edgeR package. Functional enrichment analysis was subsequently performed using the KEGG and MSigDB databases. The association between gene expression and patient survival was evaluated using Kaplan–Meier survival analysis and Cox proportional hazards regression. Diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis. In addition, the expression levels of TENT4A and TENT4B were experimentally validated in the HT29 and HCT116 colorectal cancer cell lines relative to HEK293 cells using quantitative real- time PCR (qRT-PCR).
Results: A total of 4,558 differentially expressed genes were identified (FDR < 0.05). TENT4A and TENT4B were significantly upregulated, whereas TENT5A, TENT5B, and TENT5C were significantly downregulated (FDR < 0.05). Functional enrichment analysis revealed a significant association of these genes with the RNA degradation pathway (adjusted P = 0.00015). Elevated TENT4A expression was significantly associated with poorer overall survival (log-rank P < 0.05) and demonstrated good diagnostic performance (AUC = 0.87, P < 0.0001), whereas TENT4B exhibited moderate diagnostic accuracy (AUC = 0.72, P = 0.01). Furthermore, qRT-PCR confirmed significantly increased expression of both genes in colorectal cancer cell lines compared with HEK293 cells (P < 0.05).
Conclusion: TENT4A appears to be a promising diagnostic and prognostic biomarker for colorectal cancer. Moreover, these findings provide a rationale for future functional investigations and the development of targeted therapeutic strategies involving TENT4A.
Methods: This descriptive- analytical study was conducted using RNA sequencing (RNA-Seq) data obtained from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) between tumor and normal tissues were identified using the edgeR package. Functional enrichment analysis was subsequently performed using the KEGG and MSigDB databases. The association between gene expression and patient survival was evaluated using Kaplan–Meier survival analysis and Cox proportional hazards regression. Diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis. In addition, the expression levels of TENT4A and TENT4B were experimentally validated in the HT29 and HCT116 colorectal cancer cell lines relative to HEK293 cells using quantitative real- time PCR (qRT-PCR).
Results: A total of 4,558 differentially expressed genes were identified (FDR < 0.05). TENT4A and TENT4B were significantly upregulated, whereas TENT5A, TENT5B, and TENT5C were significantly downregulated (FDR < 0.05). Functional enrichment analysis revealed a significant association of these genes with the RNA degradation pathway (adjusted P = 0.00015). Elevated TENT4A expression was significantly associated with poorer overall survival (log-rank P < 0.05) and demonstrated good diagnostic performance (AUC = 0.87, P < 0.0001), whereas TENT4B exhibited moderate diagnostic accuracy (AUC = 0.72, P = 0.01). Furthermore, qRT-PCR confirmed significantly increased expression of both genes in colorectal cancer cell lines compared with HEK293 cells (P < 0.05).
Conclusion: TENT4A appears to be a promising diagnostic and prognostic biomarker for colorectal cancer. Moreover, these findings provide a rationale for future functional investigations and the development of targeted therapeutic strategies involving TENT4A.
Keywords: Colorectal cancer, TENT4A, TENT4B, RNA-Seq, Differentially Expressed Genes, TCGA, RNA degradation, Molecular biomarker
Type of Study: Original |
Subject:
Cellular Sciences (Molecular Cells, Stem Cells)
Received: 2026/02/22 | Accepted: 2026/08/2 | Published: 2026/10/17
Received: 2026/02/22 | Accepted: 2026/08/2 | Published: 2026/10/17
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