Volume 50, Issue 1 (5-2026)
Research in Medicine 2026, 50(1): 0-0 |
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Ethics code: IR.SBMU.MSP.REC.1401.021
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Nikzad Alamshiri A, Seyedjavadi S S, Fatemeh Peyravii Ghadikolaii F, Goudarzi M. Evaluation of the Antifungal and Antibiofilm Effects of Hyde C1 Peptide on Candida albicans. Research in Medicine 2026; 50 (1)
URL: http://pejouhesh.sbmu.ac.ir/article-1-3535-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3535-en.html
Atefeh Nikzad Alamshiri 
, Sima Sadat Seyedjavadi , Fatemeh Fatemeh Peyravii Ghadikolaii , Mehdi Goudarzi
, Sima Sadat Seyedjavadi , Fatemeh Fatemeh Peyravii Ghadikolaii , Mehdi Goudarzi
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran. , sima_seyedjavadi@yahoo.com
Abstract: (17 Views)
Background and Aim: The increasing resistance of Candida albicans to commonly used antifungal agents has highlighted the need to identify new antifungal compounds. Hyde C1 peptide, derived from chicory, has previously been reported to possess antibacterial activity; however, its antifungal effects and possible mechanisms of action against C. albicans have been less investigated. This study aimed to evaluate the antifungal and antibiofilm effects of Hyde C1 peptide and investigate the potential roles of membrane damage and reactive oxygen species production.
Methods: This was an experimental, laboratory- based in vitro study. The antifungal activity of the peptide against the standard strain C. albicans ATCC 10231 and five clinical isolates was evaluated using the broth microdilution method. To assess its mechanism of action, protein leakage assay, propidium iodide staining followed by flow cytometry, ROS measurement using DCFH-DA, and biofilm inhibition assay using the crystal violet method were performed. Data were analyzed using GraphPad Prism version 8 and one-way ANOVA.
Results: Hyde C1 peptide showed antifungal activity against all tested C. albicans strains. The MIC values ranged from 8 to 32 µg/ mL, and the MFC values ranged from 16 to 64 µg/ mL. Treatment of C. albicans cells with the peptide caused a concentration- and time- dependent increase in intracellular protein leakage; protein leakage was significantly increased at 1×MIC and 2×MIC compared with the untreated control group (P < 0.001). Propidium iodide staining showed that the percentage of membrane- damaged cells reached 22.6% and 48.5% following treatment with 1×MIC and 2×MIC, respectively. Moreover, Hyde C1 increased intracellular reactive oxygen species production, with ROS-positive cells increasing to 44% and 68.2% at 1×MIC and 2×MIC, respectively. The peptide also inhibited C. albicans biofilm formation in a concentration-dependent manner. Biofilm inhibition was 34.6% at 8 µg/ mL (P < 0.05), increasing to 62.3%, 81.5%, and 87.2% at 16, 32, and 64 µg/ mL, respectively (P < 0.001).
Conclusion: Hyde C1 peptide exhibits antifungal and antibiofilm activity against C. albicans and may exert its effects through membrane damage and induction of oxidative stress.
Methods: This was an experimental, laboratory- based in vitro study. The antifungal activity of the peptide against the standard strain C. albicans ATCC 10231 and five clinical isolates was evaluated using the broth microdilution method. To assess its mechanism of action, protein leakage assay, propidium iodide staining followed by flow cytometry, ROS measurement using DCFH-DA, and biofilm inhibition assay using the crystal violet method were performed. Data were analyzed using GraphPad Prism version 8 and one-way ANOVA.
Results: Hyde C1 peptide showed antifungal activity against all tested C. albicans strains. The MIC values ranged from 8 to 32 µg/ mL, and the MFC values ranged from 16 to 64 µg/ mL. Treatment of C. albicans cells with the peptide caused a concentration- and time- dependent increase in intracellular protein leakage; protein leakage was significantly increased at 1×MIC and 2×MIC compared with the untreated control group (P < 0.001). Propidium iodide staining showed that the percentage of membrane- damaged cells reached 22.6% and 48.5% following treatment with 1×MIC and 2×MIC, respectively. Moreover, Hyde C1 increased intracellular reactive oxygen species production, with ROS-positive cells increasing to 44% and 68.2% at 1×MIC and 2×MIC, respectively. The peptide also inhibited C. albicans biofilm formation in a concentration-dependent manner. Biofilm inhibition was 34.6% at 8 µg/ mL (P < 0.05), increasing to 62.3%, 81.5%, and 87.2% at 16, 32, and 64 µg/ mL, respectively (P < 0.001).
Conclusion: Hyde C1 peptide exhibits antifungal and antibiofilm activity against C. albicans and may exert its effects through membrane damage and induction of oxidative stress.
Keywords: Hyde C1 peptide, Antifungal activity, Candida albicans, Reactive oxygen species, Biofilm, Drug resistance, Oxidative stress, Antimicrobial peptide
Type of Study: Original |
Subject:
Mucology
Received: 2026/05/10 | Accepted: 2026/07/1 | Published: 2026/10/17
Received: 2026/05/10 | Accepted: 2026/07/1 | Published: 2026/10/17
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